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In vivo 13C-NMR studies of polymer synthesis in Rhizobium meliloti M5N1 strain

✍ Scribed by Patricia Tavernier; Isabelle Besson; Jean-Charles Portais; Josiane Courtois; Bernard Courtois; Jean-Noël Barbotin

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 100 KB
Volume: 58
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19980420)58:2/3<250::aid-bit18>3.0.co;2-g

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✦ Synopsis

The use of in vivo 13 C-NMR approach for the monitoring of the synthesis of various polymers within cells of Rhizobium meliloti (M5N1 strain) is reported. Significant differences in polymer biosynthesis have been shown as a function of the metabolic state of the cells and the labeled carbon source used. Consumption of carbon source and produced glycogen was complete with mid-exponential phase harvested cells. This was not the case with stationary phase harvested cells, for which polyhydroxybutyrate synthesis was higher and gluconate synthesis was lower than the former. [1-13 C]fructose-grown cells produced more exopolysaccharide and polyhydroxybutyrate, but less ␤-(1,2) glucan and gluconate than [1-13 C]glucose-grown cells. This approach offers a suitable tool to examine the kinetics of polymer biosynthesis by Rhizobia.

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