In VitroFibrinogen Adsorption from Various Dilutions of Human Blood Plasma on Glow Discharge Modified Polyethylene
✍ Scribed by Jui-Che Lin; S.L. Cooper
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 219 KB
- Volume
- 182
- Category
- Article
- ISSN
- 0021-9797
No coin nor oath required. For personal study only.
✦ Synopsis
such as adhesion and aggregation of platelets that initiate Studies have suggested that the adsorption of fibrinogen onto a clot formation, are most likely mediated by this protein layer biomaterial's surface plays an important role in determining a instead of by the material surface itself. Hence, it is immaterial's blood compatibility. In this study, fibrinogen adsorption portant to understand the composition and organization of from various dilutions of human blood plasma was measured on this protein layer and its interrelationship with a material's various radio frequency glow discharge (RFGD) modified sursurface properties before a material with better blood comfaces. In addition, the relative binding strength of adsorbed fibrinpatibility can be developed.
ogen on these surfaces was assessed by subsequent sodium dodecyl
Many studies have shown that the composition and organisulfate (SDS) elution. More fibrinogen was adsorbed on the hydrozation of the adsorbed protein layer can be varied by numerphilic surfaces modified by an ammonia plasma, a sulfur dioxide plasma, and an ammonia plasma with propane sultone post treat-ous factors relating to the substrate, such as its hydrophoment than on the untreated polyethylene control. These three hybicity (2), sorbed water content (3), microphase separation drophilic surfaces also exhibited higher fibrinogen retention values (4), and surface chemical functionality (5). Among the hunthan the untreated polyethylene control did. The high in vitro dreds of proteins found in blood, fibrinogen has been one fibrinogen adsorption results observed on these hydrophilic surof the most widely studied. Precoated fibrinogen has been faces are closely parallel to those obtained previously in ex vivo demonstrated to enhance surface induced thrombosis (6). canine shunt experiments. Transient fibrinogen adsorption, also In addition, fibrinogen has been shown to be a key factor in known as the ''Vroman Effect,'' was not observed on the surfaces thrombosis because a specific receptor for the fibrinogen modified by the ammonia plasma and the ammonia plasma with molecule, the glycoprotein complex IIb-IIIA (GP IIb-IIIA), propane sultone post treatment. In contrast, maxima in fibrinogen is found on the platelet membrane (7). Formation of these adsorption were noticed on the surface modified by the sulfur fibrinogen-GP IIb-IIIA complexes greatly enhances platelet dioxide plasma. Despite the variations in surface chemistry, similar fibrinogen adsorption and SDS elution results were noticed on activation. a Silastic control surface and surfaces modified by various plasma Studies have suggested that the composition and conforpolymerized siloxanes.