In vitroclonal propagation of tea (Camellia sinensis(L.) O. Kuntze)

✍ Scribed by Beena Agarwal; Urmi Singh; Maitrevi Banerjee

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 738 KB
• Volume: 30
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00039995

✦ Synopsis

A system for in vitro clonal propagation has been develeped in tea plants. Shoots obtained from primary explants were induced from terminal buds and axillary buds of mature field-grown plants. Cultures were initiated from both types of explants on Murashige and Skoog (MS) medium supplemented with 10% coconut milk (CM), 200 mg 1-1 of yeast extract (YE), 1.4 p~M indoleacetic acid (IAA) and 17.8~M benzyladenine (BA). The shoot tips were multiplied on 1/2 strength MS medium containing 10% CM, 2.9 ~M IAA and 17.8 p~M BA. The larger shoots were separated after multiplication and rooted on 1/2 MS medium supplemented with 11.4p~M ascorbic acid and 34.5 ~M indolebutyric acid (IBA). A pretreatment of the plants with an aqueous solution of 493 p~M IBA greatly increased the frequency of rooting. More than 60% of the rooted plants have been transferred to soil successfully.