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In vitro uptake of amphiphilic, hydrogel nanoparticles by J774A.1 cells

✍ Scribed by Dimitris Missirlis; Jeffrey A. Hubbell

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
264 KB
Volume
93A
Category
Article
ISSN
1549-3296

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✦ Synopsis

Abstract

We here report improved synthesis and in vitro interactions of amphiphilic hydrogel nanoparticles with the macrophage cell line J774A.1. Nanoparticles comprising dispersed hydrophobic nanodomains of poly(propylene glycol) within a continuous phase of hydrophilic poly (ethylene glycol) (PEG) were prepared via inverse emulsion crosslinking polymerization, using acrylated PEG and Pluronic® F127 as macromonomer blocks. Functionality and fluorescent labeling were achieved through incorporation of reactive comonomers and a posteriori reaction with fluorescein, respectively. When introduced to a static cell culture of adhered J774A.1 macrophages, the cells internalized these hydrogel nanoparticles in a dose‐ and time‐ dependent manner through clathrin‐mediated and other pathways. Amphiphilic nanoparticle uptake was however dramatically lower than that of a model system (Fluospheres®) and similar to PEG‐coated colloids reported in the literature, which are considered “stealth.” Our findings support the potential of the nanoparticles presented here as long‐circulating drug carriers. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010

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