In-vitro-Untersuchungen zur mikrobiellen Inkorporation von Stickstoff aus [15N2]Harnstoff und [15N2]Ammoniumchlorid durch die Intestinalflora des Menschen
✍ Scribed by Stolpe, H. J. ;Heine, W. ;Bohnenstengel, C. M. ;Grütte, F.-K. ;Wutzke, K.
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 308 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0027-769X
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✦ Synopsis
6 typical bacteria species of the human intestinal flora (E. coli, Klebsiella pneumoniae, Proteus vulgaris, Streptococcus faecalis, Bacteroides fragilis, Bifidobacterium sp.) were incubated in a liquid medium for 48 h with [15N2]-urea and [15N]-ammonium chloride. The rates of [15N]-incorporation were calculated. They depend reproducible on the species examined, on the kind of the offered NPN-substance and on the amount of NPN-substance in the medium. With [15N2]-urea the minimal rate of incorporation was 3.8% (E coli) and the maximal one 95.6% (Bifidobacterium sp.). With [15N]-ammonium chloride the corresponding figures were 31.0 (Proteus vulg.) and 98.0% (Bifidobacterium sp.). The findings are discussed with regard to a possible enteral detoxification in uremic patients by bacterial utilization and elimination of urea and ammonia.