In vitro trophic effects of synthetic ACTH1–24

✍ Scribed by D. Jolink; Dr. H. A. Drexhage; R. D. van der Gaag

• Book ID: 101819819
• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 400 KB
• Volume: 2
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/cbf.290020408

✦ Synopsis

In vitro trophic effects of adrenocorticotrophinI 24 ( A C T H I 2 4 , Svnacthen) on adrenal cells were studied, using an in vitro assay system ofguinea-pig adrenal segments kept in organ culture. T w o separate methods for detecting growth activity were used, namely the measurement of thymidine kinase and a nucleic acid cytophotometric method. Synthetic ACTH was able to induce growth in the adrenal explants at very low concentrations (10-25 fg ml ' 1 . Biphasic dose-response curves were obtained, comparable to those described for other cytochemical bioassays. The principles of this assay system may allow the development of a new bioassay for the measurement ofplasnia concentrations of A C T H or antibodies mimicking the growth effect of this trophic hormone.