In vitro translatability of RNP-particles from insect eggs and embryos (Smittia spec., Chironomidae, Diptera)
✍ Scribed by Jäckle, Herbert
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 398 KB
- Volume
- 212
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Maternal messenger ribonucleoprotein (mRNP)‐particles from eggs and mRNP‐particles from embryos of the chironomid midge Smittia spec. were compared with respect to translatability in a mRNA‐dependent cell‐free translation system. The reticulocyte lysate system used was almost not stimulated by addition of native cytoplasmic mRNP‐particles from both eggs and embryos. RNP‐particles from embryos were fully translatable after partial release of proteins by high‐salt treatment. However, complete removal of proteins was necessary for translation of mRNP‐particles from eggs. Maternal mRNP‐particles of eggs consist of salt‐resistant RNP‐core proteins, which repressed translatability of the associated mRNA. The data support the hypothesis of “masked” maternal mRNA in insect embryos.