In vitro synthesis of IgM rheumatoid factor in response to STAPHYLOCOCCUS AUREUS, by lymphocytes from healthy adults

✍ Scribed by Rose Goldstein; Dr. Jacob Karsh

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 539 KB
• Volume: 29
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780291204

✦ Synopsis

Peripheral blood mononuclear cells from 20 healthy adults were tested in vitro for the production of IgM rheumatoid factor (RF) in response to Staphylococcus uureus Cowan I (SAC) or pokeweed mitogen. Fifteen of the 20 normal subjects produced 2 4 ng/ml IgM-RF (mean 2 SD 46 & 55 ng/ml) in response to SAC, compared with only 2 of 20 who produced 2 4 ng/ml IgM-RF (mean 2 SD 2 f 4 ng/ml) in response to pokeweed mitogen (P = 0.0001). Separation and reconstitution of autologous T and B cell-enriched fractions, with and without prior T cell irradiation, provided evidence for a radiosensitive T helpedinducer cell involved in the IgM-RF response to SAC in 70% of the normal subjects studied. SAC appears to be a potent stimulus of IgM-RE' production, with a cellular mechanism distinct from that of other in vitro systems.

Rheumatoid factors (RF), autoantibodies to the Fc portion of IgG, are found frequently in patients with autoimmune diseases including rheumatoid arthritis (RA), and in those with chronic infections characterized by a persistent immune response. While experimental evidence supporting a role of rheumatoid From the Rheumatic Disease Unit,