In vitro synergistic activity of PDN-IFNα and NM + IFNα combinations on fresh bone-marrow samples from multiple myeloma patients
✍ Scribed by Enrica Lepri; Dr Anna Barzi; Emma Menconi; Maria Giovanna Portuesi; Marina Liberati
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 450 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0278-0232
No coin nor oath required. For personal study only.
✦ Synopsis
The differential staining cytotoxicity (DiSC) assay was used to evaluate the in vitro sensitivity of tumour and normal bone-marrow cells from 21 multiple myeloma (MM) patients to antitumour agents methylprednisolone (PDN), nitrogen mustard (NM) and recombinant interferon a-2b (IFNa) tested singly and in the combinations PDN + IFNa and NM + IFNa. Both the PDN-IFNa and NM-IFNa associations were more efficacious than any agents used singly in reducing the percentage of myeloma cell survival. However, whereas NM, alone and in combination with IFNa, provoked a severe reduction in normal bone-marrow population, PDN and PDN+IFNa induced an increase percentage survival of normal bone-marrow cells. These findings indicate that, at least in vitro, the PDN-IFNa combination exerts a great antitumour effect which is not associated with a relevant cytotoxic activity on normal myeloid cells.