In Vitro Selection of RNA Aptamers Carrying Multiple Biotin Groups in the Side Chains
β Scribed by Ito, Yoshihiro; Suzuki, Akinori; Kawazoe, Naoki; Imanishi, Yukio
- Book ID
- 127231249
- Publisher
- American Chemical Society
- Year
- 2001
- Tongue
- English
- Weight
- 130 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1043-1802
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## Abstract __SELEX was used to create an RNA aptamer targeted to protein tyrosine phosphatase 1B (PTP1B), an enzyme implicated in type 2 diabetes, breast cancer and obesity. We found an aptamer that strongly inhibits PTP1B in vitro with a__ K__~i~ of less than 600 pM. This slowβbinding, highβaffin