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In vitro selection of a DNA aptamer targeted against Shigella dysenteriae

✍ Scribed by Duan, Nuo; Ding, Xiaoying; Wu, Shijia; Xia, Yu; Ma, Xiaoyuan; Wang, Zhouping; Chen, Jie

Book ID
121005709
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
371 KB
Volume
94
Category
Article
ISSN
0167-7012

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In vitro selection of a DNA aptamer targ
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✍ Duan, Nuo; Ding, Xiaoying; Wu, Shijia; Xia, Yu; Ma, Xiaoyuan; Wang, Zhouping; Ch πŸ“‚ Article πŸ“… 2013 πŸ› Elsevier Science 🌐 English βš– 371 KB

To identify DNA aptamers demonstrating binding specificity for Shigella dysenteriae, a whole-bacterium Systemic Evolution of Ligands by Exponential enrichment (SELEX) method was applied to a combinatorial library of single-stranded DNA (ssDNA) molecules. After several rounds of selection using S. dy

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Listeria monocytogenes is a pathogenic bacterium capable of causing severe disease leading to high hospitalization and case fatality rates. Here, we report the use of Systemic Evolution of Ligands by Exponential Enrichment (SELEX) to screen DNA aptamers that recognize L. monocytogenes with high affi