In vitro scavenging activity for reactive oxygen species by N-substituted indole-2-carboxylic acid esters

✍ Scribed by Irena Kruk; Hassan Y. Aboul-Enein; Teresa Michalska; Krzysztof Lichszteld; Katarzyna Kubasik-Kladna; Sureyya Ölgen

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 272 KB
• Volume: 22
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.974

✦ Synopsis

Abstract

The hydroxyl radical (HO^•^)‐ and superoxide anion radical (O)‐scavenging activity, as well as the singlet oxygen (^1^O~2~)‐quenching property of N‐substituted indole‐2‐carboxylic acid esters (INDs) were investigated by deoxyribose degradation assay, a chemiluminescence method and the electron spin resonance (ESR) spin‐trapping technique. This novel group of compounds was developed as a search for cyclooxygenase‐2 (COX‐2)‐selective enzyme inhibitors. The results obtained demonstrated that of the 16 compounds examined, five inhibited light emission from the superoxide anion radical (O)–DMSO system by at least 60% at a concentration of 1 mmol/L, nine prevented the degradation of deoxyribose induced by the Fenton reaction system (range 3–78%) or scavenged hydroxyl radicals (HO^•^) directly (range 8–93%) and 14 showed the ^1^O~2~‐quenching effect (range 10–74%). These results indicate that majority of the indole esters tested possess the ability to scavenge O^−^~2~ and HO radicals and to quench ^1^O~2~ directly, and consequently may be considered effective antioxidative agents. Copyright © 2007 John Wiley & Sons, Ltd.