In vitro response and pedigree analysis for somatic embryogenesis of long-day photoperiod adapted soybean
✍ Scribed by L. N. Tian; D. C. W. Brown; H. Voldeng; J. Webb
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 287 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
Northern-adapted soybean cultivars were screened for their ability to form somatic embryos in vitro in response to exposure to 180 ~tM 2,4-dichlorophenoxyacetic acid in a modified Murashige & Skoog (1962) medium containing B5 (Gamborg et al. 1968) organics and 43.5 mM sucrose. The 20 cultivars formed between 1 and 7 embryos per cotyledon with between 18% and 98% of the cotyledons responding. The response was genotype dependant. Three ancestral lines, '840-7-3', 'A.K. Harrow' and 'Mandarin' were prominent in the genetic background of long-photoperiod adapted lines and showed a high degree of somatic embryogenesis.