In vitro reconstitution of nitrate reductase activity of the Neurospora crassa mutant nit-1: Specific incorporation of molybdopterin
โ Scribed by Steven Kramer; Robert V. Hageman; K.V. Rajagopalan
- Book ID
- 115706284
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 768 KB
- Volume
- 233
- Category
- Article
- ISSN
- 0003-9861
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๐ SIMILAR VOLUMES
Molybdenum cofactor (MoCo) mutants of Neurospora crassa lack both NADPH-nitrate reductase and xanthine dehydrogenase activity. In vivo and in vitro studies to further characterize these mutants are now reported. The MoCo mutants nit-9A and nit-9B are capable of growing, albeit poorly, with nitrate a
Neurospora crassa nmr-1 mutants, selected on the basis of their sensitivity to chlorate in the presence of glutamine, have elevated levels of the nitrate assimilation enzymes, NADPH-nitrate reductase and NAD(P)H-nitrite reductase. Immunoelectrophoretic determinations show that the higher nitrate red