✦ LIBER ✦

In vitro protein expression: an emerging alternative to cell-based approaches

✍ Scribed by Mingyue He

Publisher: Elsevier
Year: 2011
Tongue: English
Weight: 76 KB
Volume: 28
Category: Article
ISSN: 1871-6784
DOI: 10.1016/j.nbt.2010.08.011

No coin nor oath required. For personal study only.

✦ Synopsis

Protein expression remains a bottleneck in the production of proteins. Owing to several advantages, cell-free translation is emerging as an alternative to cell-based methods for the generation of proteins. Recent advances have led to many novel applications of cell-free systems in biotechnology, proteomics and fundamental biological research. This special issue of New Biotechnology describes recent advances in cell-free protein expression systems and their applications.

📜 SIMILAR VOLUMES

Valproic acid: A viable alternative to s

Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

✍ Gaurav Backliwal; Markus Hildinger; Ivan Kuettel; Fanny Delegrange; David L. Hac 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 198 KB

## Abstract Various DNA methyl transferase inhibitors (iDNMTs) and histone deacetylase inhibitors (iHDACs) were screened for their ability to enhance transient gene expression (TGE) in Human Embryonic Kidney 293‐EBNA (HEK293E) cells. The effects in HEK293E cells were compared to those in Chinese Ha

A proteomic approach to mimic fibrosis d

A proteomic approach to mimic fibrosis disease evolvement by an in vitro cell line

✍ Johan Malmström; Gunilla Westergren-Thorsson; György Marko-Varga 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 206 KB

A New Approach for Adipose Tissue Regene

A New Approach for Adipose Tissue Regeneration Based on Human Mesenchymal Stem Cells in Contact to Hydrogels—an In Vitro Study

✍ Kirsten Peters; Achim Salamon; Sandra Van Vlierberghe; Joachim Rychly; Michael K 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 429 KB 👁 2 views

Establishment of permanent astroglial ce

Establishment of permanent astroglial cell lines, able to differentiate in vitro, from transgenic mice carrying the polyoma virus large T gene: An alternative approach to brain cell immortalization

✍ E. Galiana; I. Borde; P. Marin; M. Rassoulzadegan; F. Cuzin; F. Gros; Dr. P. Rou 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 976 KB

## Abstract Permanent untransformed cell lines have been established from the cerebral cortex of transgenic mice that carry the polyoma virus large T gene. The immortalized cells described here synthesize laminin and neural cell adhesion molecules and induce primary neurons to develop neuritic proc

Chitosan scaffolds for in vitro buffalo

Chitosan scaffolds for in vitro buffalo embryonic stem-like cell culture: An approach to tissue engineering

✍ Wah W. Thein-Han; Yindee Kitiyanant 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 669 KB

## Abstract Three‐dimensional (3D) porous chitosan scaffolds are attractive candidates for tissue engineering applications. Chitosan scaffolds of 70, 88, and 95% degree of deacetylation (% DD) with the same molecular weight were developed and their properties with buffalo embryonic stem‐like (ES‐li

What are the best host cells for express

What are the best host cells for expression of functional proteins for in vitro analysis? What are the vectors of choice for expression? What can we learn from site-specific mutagenesis coupled to in vitro studies on expressed proteins?

📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 97 KB 👁 1 views