In vitro propagation ofClianthus formosus(Sturt's desert pea) an Australian native legume
✍ Scribed by Acram M. Taji; Richard R. Williams
- Book ID
- 104620868
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 715 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
✦ Synopsis
Hypocotyl and cotyledon segments of Clianthus formosus were cultured on a modified deFossard medium M supplemented with cytokinins. 62% of cultures on medium with 20 #M BA produced callus which subsequently gave rise to shoots. 40% of shoots excised from callus produced roots after transfer to auxin-rich media (20/~MNAA or 10/~M IBA + 10/~M NAA). Root production was enhanced following a 7-day dark treatment. 32% of nodes from mature plants produced multiple shoots on 2 laM BA + 2/~M KIN. 30% of these shoots rooted on medium without hormones. 70% of rooted plantlets were successfully transferred to potting medium and glasshouse conditions. A period of cold treatment (10 days at 5 °C) reduced vitrification from 68 to 22% of cultures.