In vitro propagation of prairie gentian

✍ Scribed by Peter Semeniuk; R. J. Griesbach

• Book ID: 104618994
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 622 KB
• Volume: 8
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00040952

✦ Synopsis

Explants of shoot tips, internodal stem sections, and leaf segments of Lisianthus, Eustoma grandiflorum (Griseb.) Schinners, 'Dwarf Purple' were cultured in vitro on modified Murashige and Skoog (MS) media. Explants of shoot tips and internodal stem sections developed into multiple shoots, whereas, leaf segments turned chlorotic on a medium supplemented with 3mgl -t benzyladenine (BA) and 0.2mgl i naphthalene acetic acid (NAA). Shoot proliferation was obtained on shoot tips and leaf segments with 3 mgl-] BA, but internodal stem sections became necrotic and died on this medium. Rooting was induced in cultures with multiple shoots by subculturing explants on a half-strength MS medium supplemented with 2mg1-1 indole-3-acetic acid (IAA). Rooted plantlets were successfully transferred to soil.