In vitro production of polyhydroxyalkanoates: achievements and applications

✍ Scribed by Nicholas Thomson; Ipsita Roy; David Summers; Easan Sivaniah

• Book ID: 102315911
• Publisher: Wiley (John Wiley & Sons)
• Year: 2009
• Tongue: English
• Weight: 236 KB
• Volume: 85
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.2299

✦ Synopsis

Abstract

The mechanisms of polyhydroxyalkanoate (PHA) production have been studied for over half a century. However, despite numerous improvements in the control of monomer composition, genetically‐engineered host organisms, fermentation strategies and polymer recovery processes they remain uncompetitive compared with petrochemical plastics. Recently, interest has developed in the enzyme‐catalysed production of PHAs in vitro. This has allowed the study of enzyme kinetics and properties, and represents another strategy for the economic production of PHAs on the industrial scale. It also presents an opportunity to coat other materials in thin films of PHA so as to modify the surface properties. In vitro production offers advantages over in vivo methods as it enables greater control over monomer composition and molecular weight, does not require a biomass‐accumulation phase, simplifies downstream processing and can utilise a wider range of monomeric subunits. Copyright © 2009 Society of Chemical Industry