In vitro production of granulocyte–macrophage colony-stimulating activity by murine bone marrow and spleen following vinblastine administration IN VIVO

✍ Scribed by Douglas E. Williams; David S. Chervinsky; Frank R. Orsini; Cameron K. Tebbi; John E. Fitzpatrick

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 620 KB
• Volume: 5
• Category: Article
• ISSN: 0278-0232
• DOI: 10.1002/hon.2900050105

✦ Synopsis

The production of granulocyte-macrophage colony-stimulating activity (CSA) by isolated murine femur shafts, non-dispersed bone marrow and spleens was assessed following administration of Vinblastine (VLB). These organs were removed from 2 h to 10 days post-VLB and allowed to condition endotoxin-free medium for 48 h. CSA activity was assessed using a soft agar cloning system. The data demonstrate that CSA elaboration was maximal 24 h post-VLB, corresponding to the nadir of bone marrow GM-CFC, and 6 h after splenic reached a minimum. No relationship between peripheral blood granulocyte count or serum endotoxin levels were observed.

KEY WORDS Granulocyte-macrophage colony-stimulating activity Vinblastine

Supported in part by a grant from the Association for the Research of Childhood Cancer (AROCC), Buffalo, NY. D.E.W. was an AROCC pre-doctoral fellow during these studies.