In vitro plant regeneration from unpollinated ovaries of Allium chinense
โ Scribed by Amadou A. Bah; Zhi-Dan Wang; Xiao-Qing Wang; Miao-Miao Yan; Yeong-Cheol Um; Zhen Xu; De-Ping Guo
- Book ID
- 119366439
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 546 KB
- Volume
- 147
- Category
- Article
- ISSN
- 0304-4238
No coin nor oath required. For personal study only.
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Haploid plants have been obtained from unpollinated ovules of onion cultured in vitro as here reported for the first time. Callus was not formed at any step of the process and the young developing embryos sprouted by splitting the ovules. From a total of about 26 000 cultured ovules, 13 embryos were
Shoots were produced from kurrat seedling and mature plant explants cultured in Murashige and Skoog medium (MS) alone or supplemented with 4.4 ~tM benzyladenine (BA). Shoots were also produced from explants through a two-step procedure. Regenerated shoots were induced to form roots on MS medium with