In vitro plant regeneration from leaf base and mesocotyl cultures ofEchinochloa colona

✍ Scribed by S. Samantaray; G. R. Rout; P. Das

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 848 KB
• Volume: 40
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00041116

✦ Synopsis

Leaf base and mesocotyl explants derived from in vitro-grown seedlings of Echinochloa colona were cultured on Murashige and Skoog's (MS) medium containing various concentrations of benzyladenine (BA), anaphthaleneacetic acid (NAA) and kinetin. Leaf base and mesocotyl segments exhibited optimal morphogenetic response by using 6.66 ~tM BA with 2.68 laM NAA. Induction of rooting from regenerated shoots was readily achieved in half strength MS medium without organics and growth regulators. Histological studies revealed the sequence of shoot bud regeneration in the monocot system. The in vitro-raised plants were established in chromite minewaste.