In vitro photosensitization II. An electron microscopy study of cellular destruction with mono-L-aspartyl chlorin e6 and photofrin II
✍ Scribed by Dr. W. Gregory Roberts; L.-H. L. Liaw; Michael W. Berns
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 870 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0196-8092
No coin nor oath required. For personal study only.
✦ Synopsis
Primary sites of subcellular destruction with photofrin I1 (PfI I) and mono-L-aspartyl chlorin e6 (MACE) were determined by transmission electron microscopy (TEM). Potorous tridactylus (PTK2) cells were grown in Rose chambers and incubated for 24 hr with a sensitizer concentration sufficient to provide 100% mortality. Cells were irradiated with laser light and fixed and processed for electron microscopy at various times post-irradiation. The results indicate that PfI I initially destroys mitochondria, whereas MACE destroys lysosomes. Both conclusions are consistent with fluorescence subcellular localization studies.