✦ LIBER ✦

In-vitro modification of membrane- receptors on cells of the mononuclear phagocyte system

✍ Scribed by Marian Ridley; J. L. Turk; P. Badenoch-Jones

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 677 KB
Volume: 127
Category: Article
ISSN: 0022-3417
DOI: 10.1002/path.1711270403

No coin nor oath required. For personal study only.

✦ Synopsis

EA and EAC receptors have been studied on non-elicited and paraffin-induced macrophages, under a variety of culture conditions in vitro, for up to 7 days. A large decrease in the number of macrophages showing EAC receptors was found after treatment of the cells with BCG, but not "inert" particles such as latex and zymosan. This was reversible by 7 days. In the presence of a toxic material, Al(OH)3, both EA and EAC receptors were partially lost. The results obtained have been related to previous results obtained with cryostat sections of human leprosy skin lesions.

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E, EA and EAC rosetting techniques and Ig fluorescence were used in a study of receptor sites in cryostat sections of lesions through the spectrum of leprosy, and for comparison in some other mycobacterial and granulomatous lesions. Anti-C3, and trypsin were used as blocking agents. Lymphocytes in b