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In vitro model of stromal and epithelial immortalized endometriotic cells

✍ Scribed by Mariarosaria Boccellino; Lucio Quagliuolo; Annarita Verde; Raffaele La Porta; Stefania Crispi; Maria Teresa Piccolo; Antonio Vitiello; Alfonso Baldi; Pietro Giulio Signorile

Publisher: John Wiley and Sons
Year: 2012
Tongue: English
Weight: 641 KB
Volume: 113
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.24000

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✦ Synopsis

Abstract

Endometriosis is a relatively common chronic gynecologic disorder that usually presents with chronic pelvic pain or infertility. It results from implantation of endometrial tissue outside the uterine cavity. Despite its frequency and its impact on quality of life, the understanding of pathogenesis of endometriosis remains incomplete and its treatment remains controversial. In this work, we established a suitable in vitro model system of immortalized human endometriotic cell line taking advantage of the human telomerase reverse transcriptase. The results demonstrate that these cells retain the natural characteristics of endometrial cells in term of phenotype and of functional expression of estrogen and progesterone receptors, without chromosomal abnormalities. In conclusion, these cells are potentially useful as an experimental model to investigate endometriosis biology. J. Cell. Biochem. 113: 1292–1301, 2012. © 2011 Wiley Periodicals, Inc.

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