In vitro model for frontal sinus obliteration with bioactive glass S53P4

✍ Scribed by Peltola, Matti J. ;Suonp��, Jouko T. K. ;Andersson, H. ;M��tt�nen, Heli S. ;Aitasalo, Kalle M. J. ;Yli-Urpo, Antti ;Laippala, Pekka J.

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 207 KB
• Volume: 53
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(2000)53:2<161::aid-jbm5>3.0.co;2-3

✦ Synopsis

An in vitro model was used to investigate the behavior of a massive frontal sinus obliteration with bioactive glass S53P4 (BG) for clinical purposes. Two sizes of granules (0.63-0.8 mm or 0.8 -1.0 mm) in 16 separate BG amounts, weight 25 g, were tested both in simulated body fluid (SBF) and a buffer containing trishydroxymethyl aminomethane citric acid (TRIS-c.a) in standard conditions. The dissolution of silicon (Si) and phosphate (P) was detected with direct current plasma atom emission spectroscopy (DCP-AES) monthly up to 6 months. The BG masses were scanned by computer tomography (CT) and the scans analyzed by Region of Interest (ROI) technique. Calcium phosphate (CaP)-and silica (Si)-gel-layers were studied by scanning electron microscopy (SEM) at 1, 3, and 6 months. Cumulative loss of Si and P was stronger in TRIS -c.a than in SBF (p < 0.0001), and it was higher with smaller than with larger granules in both solutions (p < 0.0001).This was shown correspondingly by the decrease in Hounsfield units (HU) by ROI analysis (p < 0.0001). In SBF-soaked BG masses, the CaP-layer occurred on the uppermost granules, and in TRIS-c.a at 3-6 months, on the granules in the center and lower parts. The decrease of HU seems to reveal indirectly the resorption of BG.