In vitro measurement of chemosensitivity of human small cell lung and gastric cancer cell lines toward cell cycle phase-nonspecific agents under the clinically equivalent area under the curve
✍ Scribed by Yoshihiro Mitsuhashi; Makoto Lnaba; Yuichi Sugiyama; Tomowo Kohayashi
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 611 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
Background. Based on the previous finding that cell killing effects of cell cycle phase-nonspecific agents depend on the concentration-time product (C X T) or the area under the curve (AUC), the authors investigated in vitro cytotoxic effects of nimustine hydrochloride (ACNU) and mitomycin C (MMC) under an experimental condition in which the assay AUC was equivalent to their clinically achievable AUC.
Methods. The cytotoxic effects of these agents on human cancer cell lines, consisting of 9 small cell lung carcinomas (SCLC) and 10 gastric cancers, were measured by a tetrazolium-based colorimetric assay (MTT assay).
Results. These cell lines individually responded to ACNU and MMC in this assay condition. When the authors considered 60% or greater cell kill to be effective, the in vitro response rates of SCLC to ACNU and MMC were 22% (two of nine carcinomas) and 67% (six of nine carcinomas), respectively. The response rates of gastric cancer to ACNU and MMC were 10% (1 of 10 carcinomas) and 40% (4 of 10 carcinomas), respectively. Except for the response of SCLC to ACNU, these in vitro response rates corresponded well to the clinical rates (SCLC to ACNU