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In vitro labeling of human polymorphonuclear leukocytes with99mTc

✍ Scribed by Henning Kelbaek; Jan Fogh; Jens Elmgreen

Publisher
Springer
Year
1984
Tongue
English
Weight
365 KB
Volume
9
Category
Article
ISSN
0340-6997

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✦ Synopsis

Preliminary experience with 99mTc labeling of isolated polymorphonuclear leukocytes is reported. Leukocytes efficiently purified from 100 ml peripheral blood were labeled in vitro with 99mTc after pretreatment with small amounts of a stannous agent to reduce pertechnetate. A labeling yield of 1.3 mCi equivalent to 12.5% of the added dose of 99mTc and a cell binding efficiency of more than 90% was achieved under sterile conditions. Viability assays showed no influence of the labeling procedure on cell function. The technique may prove a valuable alternative for assessment of local leukocyte accumulation by scintigraphy.

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✍ Henning KelbΓ¦k; JΓΈrgen Linde; Steen Levin Nielsen πŸ“‚ Article πŸ“… 1988 πŸ› Springer 🌐 English βš– 236 KB

A technique for labeling leukocytes with 99mTc using hexamethylpropylene-amineoxime (HMPAO) was evaluated in vitro. The labeling procedure resulted in a cell bound fraction of radioactivity of 56% after 99mTc incubation and 96% after 1 washing of cells. In the final cell suspension 84% of the radioa