In vitro kinetics of the rat brain succinate dehydrogenase inhibition by hexachlorophene

✍ Scribed by V. Lokanatha; P. Sailaja; W. Rajendra

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 82 KB
• Volume: 13
• Category: Article
• ISSN: 1095-6670
• DOI: 10.1002/(sici)1099-0461(1999)13:6<303::aid-jbt3>3.0.co;2-3

✦ Synopsis

Brain succinate dehydrogenase (SDH) activity was inhibited by in vitro hexachlorophene (HCP) with a half inhibitory concentration (IC 50 ) of 0.65 ‫‬ 10 ‫3‬ M. The HCP exerted noncompetitive inhibition at 0.5 mM (ϽIC 50 ), uncompetitive inhibition at 0.65 mM (IC 50 ), and mixed-type inhibition at 0.8 mM (ϾIC 50 ) on SDH activity. The brain SDH demands more energy of activation (DE) in the presence of HCP. The ionizable groups of SDH such as the sulfhydral group of cysteine and ␣-amino groups of cysteine were not altered qualitatively in the presence of HCP.