In vitro karyotype evolution and cytogenetic instability in the non-tumorigenic human breast epithelial cell line HMT-3522
β Scribed by Kirsten Vang Nielsen; Mogens Winkel Madsen; Per Briand
- Book ID
- 113253476
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 766 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0165-4608
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π SIMILAR VOLUMES
The objective of this study was to determine whether microsatellite instability (MSI) and loss of heterozygosity (LOH) are involved in the immortalization of human breast epithelial cells (HBECs) in vitro and in the early stages of their transformation by benzo[a]pyrene (BP) and 7,12-dimethylbenz[a]
The immortalized, nontumorigenic human breast epithelial cell line HMT-3522 has been used as a model for premalignant and, eventually, malignant development. During cultivation, the karyotype evolution was followed. At an early stage, a very long constant phase showed a near-diploid karyotype, with