In vitro infection of cell lines with HTLV-I and SIVmac results in altered intracellular free calcium concentration and increased membrane polarization
✍ Scribed by K. A. Wright; R. G. Olsen
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- French
- Weight
- 432 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
This study was designed to determine whether retrovirus infection alters basal concentrations of intracellular free calcium, the magnitude of intracellular free calcium change upon receptor-mediated cell-stimulation, and cell membrane polarization. Two retrovirus-infected cell lines were utilized: HTLV-I-infected MT-2 cells and SIV-infected H-9 cells. Uninfected H-9 cells were used as controls. The results show that the retrovirally-infected cells had an increased basal concentration of intracellular free calcium when compared with the control cells and also a reduced magnitude of intracellular free calcium response to receptor-mediated cell stimulants. The retrovirally-infected cell membranes were also hyperpolarized when compared with the control cells.