In vitro induction of tetraploid plants from colchicine-treated diploid daylily callus

✍ Scribed by C. H. Chen; Yvonne C. Goeden-Kallemeyn

• Book ID: 104617720
• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 344 KB
• Volume: 28
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00038937

✦ Synopsis

Daylily plantlets with tetraploid or octaploid chromosome numbers were induced from colchicine-treated diploid callus (2n = 22). Callus tissue of the daylily Hemeroca/lisfluva L. was placed on a morphogenetic induction medium, a modified MS formula supplemented with 1 mg 2,4-D and 1 mg kinetimliter. plus 0, 10, 20, or 40 mgjliter colchicine in the dark at 12'C for 3 days and recuperated for 1 week under the same environmental conditions except that colchicine was left out. The calluses were then returned to the normal growth temperature 25 C. Ploidy was identified by chromosome counts of squashed root-tip cells collected from the newly potted plantlets and later by measurements of stomates and pollen grains. Over 50% of the plants initiated from the colchicine-treated calluses were completely tetraploid.

All of the plantlets differentiated from untreated callus had a diploid number of 22. Of the 3 colchicine levels applied, the 20 mg/liter treatment appeared to be the most effective in production of tetraploidy.