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In vitro growth of Trypanosoma musculi: Requirements of cells and serum free culture medium

✍ Scribed by P. Vincendeau; B. Guillemain; S. Daulouede; C. Ripert

Book ID
115945295
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
311 KB
Volume
16
Category
Article
ISSN
0020-7519

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## Abstract A serum‐free medium for serial culture of baby hamster kidney cell line 21 (BHK‐21) as container‐adherent cells was developed. The medium is a 1:1 (v/v) mixture of Dulbecco's modified Eagle's medium and Ham's F‐12 medium supplemented with fibroblast growth factor, fibronectin, insulin,