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In vitro generation of tumour-specific lymphocyte reactivity to colonic carcinoma cells

✍ Scribed by Maria Kuppner; Stephen Wilkinson; Elizabeth Casson; Oleg Eremin

Book ID
104660410
Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
623 KB
Volume
25
Category
Article
ISSN
0340-7004

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✦ Synopsis

Purified tumour cells and normal mucosa cells from fresh human colorectal cancer resection specimens, and T-cell-enriched autologous peripheral blood lymphocytes, were mixed in short-term (6 day) mixed lymphocytetumour cell (MLTC) microcultures. Lymphocyte stimulation was measured by 3H-thymidine uptake, and a stimulation index (SI = [lymphocytes vs tumour cells (cpm)tu- mour cells (cpm)]/[lymphocytes (cpm)]) >3 was regarded as significant. Significant lymphocyte reactivity was found in 10/15 patients with colon carcinoma. However, 1 patient with autologous tumour reactivity, also showed significant stimulation against autologous normal mucosa cells, suggesting tumour-associated reactivity. Maximum stimulation occurred most frequently at a lymphocyte:tumour cell ratio of 2:1 and with nylon woot-passaged lymphocytes.

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Human tumour—lymphocyte interaction in vitro. V. Comparison of the reactivity of tumour-infiltrating, blood and lymph-node lymphocytes with autologous tumour cells
✍ B. M. Vose; F. Vánky; E. Klein 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 609 KB

## Abstract Lymphocytes prepared from the blood, tumour‐draining lymph node and tumour were tested for immune reactivity with freshly isolated autologous tumour cells from biopsies. Reactivity was assessed by the autologous tumour stimulation assay and by lymphocytotoxicity. Activity was found in 6