In vitro fertilization and cleavage capability of bovine follicular oocytes classified by cumulus cells and matured in vitro
β Scribed by Y. Shioya; M. Kuwayama; M. Fukushima; S. Iwasaki; A. Hanada
- Book ID
- 118378085
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 416 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0093-691X
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## Abstract Bovine follicular oocytes cultured in vitro in Ham's Fβ12 based medium for 27 hours were transferred to the oviducts of rabbits pretreated with 75 IU hCG 24 hours previously. Frozenβthawed bull spermatozoa were inseminated into the uterus of rabbits 0, 2, 4, 6, 9, and 12 hours before oo
## Abstract Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15β18 hr of sper