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In vitro fertilization and cleavage capability of bovine follicular oocytes classified by cumulus cells and matured in vitro

✍ Scribed by Y. Shioya; M. Kuwayama; M. Fukushima; S. Iwasaki; A. Hanada


Book ID
118378085
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
416 KB
Volume
30
Category
Article
ISSN
0093-691X

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## Abstract Bovine follicular oocytes cultured in vitro in Ham's F‐12 based medium for 27 hours were transferred to the oviducts of rabbits pretreated with 75 IU hCG 24 hours previously. Frozen‐thawed bull spermatozoa were inseminated into the uterus of rabbits 0, 2, 4, 6, 9, and 12 hours before oo

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## Abstract Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15–18 hr of sper