Updated and expanded by nearly fifty percent, this comprehensive new edition builds on its successful and popular predecessor, while retaining the practical features that made the first edition such an essential guide to IVF. The new edition describes the most recent additions to the range of current assisted reproductive technology (ART) clinical treatments, including the use of testicular and epididymal sperm, blastocyst stage transfer, and new perspectives in cryobiology and cryopreservation techniques. The authors have incorporated the very latest laboratory techniques and protocols, with an even greater emphasis on quality control to provide an indispensable and practical account of the latest IVF techniques. The introductory chapters covering the scientific background that underpins effective laboratory practice have been substantially expanded to include the most recent information available from research in mammalian systems into the molecular biology of oogenesis, oocyte maturation, and early embryo metabolism. This new edition distills a wealth of practical and scientific detail for the benefit of all IVF practitioners.