In vitro fenoprofenyl–coenzyme a thioester formation: Interspecies variations

✍ Scribed by A. Soraci; E. Benoit

• Book ID: 102072202
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 463 KB
• Volume: 7
• Category: Article
• ISSN: 0899-0042
• DOI: 10.1002/chir.530070707

✦ Synopsis

In vitro coenzyme A thioester formation from (-)-(Rbfenoprofen (FPF) and palmitic acid has been studied using liver microsomes from rat, guinea pig, sheep, and dog. In every species with both palmitic acid or (-)-(Rbfenoprofen, the Lineweaver-Burk plot was linear in the substrate concentration range used and as a consequence agrees with the involvement of only one isoenzyme (or different isoenzymes of similar K, values). The V, , values for the thioesterification of (-)-(R)-fenoprofen present large species variations from 2.1 ? 1.0 with sheep liver microsomes to 60.6 ? 11 nmoYnidmg with dog liver microsomes. These values statistically sigdicantly correlate (r = 0.94) to the V , values observed when palmitic acid was used as a substrate. Furthermore palmitic acid inhibited (-)-(R)-fenoprofen-CoA formation in the same extent in all animal species. The stereoselectivity of the thioesterification was also species dependent. o 1995 Wiey-Liss, h c .