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In vitro expression and enzyme activity analysis of mutant human cytomegalovirus DNA polymerases

✍ Scribed by Sophie Alain


Book ID
117771592
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
87 KB
Volume
12
Category
Article
ISSN
1386-6532

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Cytomegalovirus (CMV) isolates were investigated by restriction enzyme analysis. A method for extraction of cellular CMV DNA is described which uses CMV DNA released into the Hirt supernatant after precipitation of cellular DNA. CMV DNA is further purified by ultracentrifugation in neutral sucrose g