𝔖 Bobbio Scriptorium
✦   LIBER   ✦

In vitro evaluation of degradation and cytotoxicity of a novel composite as a bone substitute

✍ Scribed by Liu, Bai-Shuan ;Yao, Chun-Hsu ;Chen, Yueh-Sheng ;Hsu, Shan-Hui

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
123 KB
Volume
67A
Category
Article
ISSN
0021-9304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The purpose of this study was to prepare and evaluate in vitro the feasibility and cytocompatibility of a novel composite (GGT) as a large defect bone substitute. The composite is tricalcium phosphate ceramic particles combined with genipin crosslinked gelatin. After soaking the GGT composites in Ringer solutions at 37°C for 7, 14, 28, 42, 56, and 84 days, the in vitro biologic degradation rate and biocompatibility were determined. Substances released from soaked GGT composites were analyzed with an ultraviolet visible light spectrophotometer. In addition, the solution soaking the GGT was co‐cultured with osteoblasts to determine whether or not the released substances from GGT could facilitate the growth of bone cells. After they had been cultured for 2 days, the osteoblasts were tested for differentiation and proliferation by alkaline phosphatase (ALP) activity and a MTT assay. Results indicate that the concentration of the genipin solution is a critical factor in deciding the crosslinking degree of the GGT composite. Complete crosslinking reaction in the GGT composite occurred when 0.5 wt % of genipin had been added. Cytotoxic testing revealed that 80 ppm of the genipin in the culture medium served as the level over which cytotoxicity to osteoblasts could be produced. In addition, we found that gelatin and calcium continuously were released from the GGT composite in the soaking solution, which promoted differentiation and proliferation of the osteoblasts. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1163–1169, 2003

📜 SIMILAR VOLUMES

In vitro evaluation of samarium (III) ox
In vitro evaluation of samarium (III) oxide as a bone substituting material
✍ H. M. T. U. Herath; L. Di Silvio; J. R. G. Evans 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 649 KB

## Abstract The biocompatibility of natural samarium (III) oxide, which has previously been used for treatment in bone‐related diseases was determined as a first step in its evaluation as a bone implant material. Assessment for 28 days using osteoblast‐like cells revealed no indications of cytotoxi

Fabrication and evaluation of a new comp
Fabrication and evaluation of a new composite composed of tricalcium phosphate, gelatin, and Chinese medicine as a bone substitute
✍ Chun-Hsu Yao; Hung-Ming Tsai; Yueh-Sheng Chen; Bai-Shuan Liu 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 865 KB

## Abstract This study investigates the biological effects of traditional Chinese medicines on the activities of bone cells using rat bone cells. Then, a mixture of a GGT composite, that is, a novel biodegradable composite containing genipin crosslinked gelatin and tricalcium phosphate, and traditi

A novel bone substitute composite compos
A novel bone substitute composite composed of tricalcium phosphate, gelatin and drynaria fortunei herbal extract
✍ Guo-Chung Dong; Hueih Min Chen; Chun-Hsu Yao 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 686 KB

## Abstract The Chinese herb, Gu‐Sui‐Bu (GSB) (__Drynaria fortunei__ J. Sm.) has been anecdotally reported to enhance bone healing. We had previously confirmed __in vitro__ the efficacy and safety of GSB in bone healing, and showed that it influenced both osteoblast and osteoclast activity. For cli

Evaluation of DBM/AM composite as a graf
Evaluation of DBM/AM composite as a graft substitute for posterolateral lumbar fusion
✍ Qing-Qing Qiu; Mei-Shu Shih; Kimberly Stock; Teri Panzitta; Patricia A. Murphy; 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 586 KB

## Abstract Demineralized bone matrix (DBM) has been investigated as a bone graft substitute for spinal fusion with less morbidity. Various carriers have been added to DBM to enhance its handling characteristics. This study investigates the spinal fusion induced by a composite of DBM and acellular

Evaluation of a resorbable,in situ setti
Evaluation of a resorbable,in situ setting bone substitute in a sheep model
✍ Niedhart, Christopher ;Maus, Uwe ;Piroth, Werner ;Miltner, Oliver ;Schmidt-Rohlf 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 650 KB

## Abstract The gold standard for bone substitution is the autologous bone graft, but because of its limited supply and the associated morbidity, the search for synthetic alternatives is necessary. A new __in situ__ setting tricalcium phosphate cement was implanted in a trepanation defect (9.4 mm d

In vitro biological evaluation of β–TCP/
In vitro biological evaluation of β–TCP/HDPE—A novel orthopedic composite: A survey using human osteoblast and fibroblast bone cells
✍ S. SH. Homaeigohar; M. A. Shokrgozar; A. Khavandi; A. Yari Sadi 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 417 KB

## Abstract Beta‐tricalcium phosphate reinforced high density polyethylene (β‐TCP/HDPE) was prepared to simulate bone composition and to study its capacity to act as bone tissue. This material was produced by replacing the mineral component and collagen soft tissue of the bone with β‐TCP and HDPE,