The free energy–reaction coordinate prof
✍
Karel Martinek; A. A. Klyosov; N. F. Kazanskaya; I. V. Berezin
📂
Article
📅
1974
🏛
John Wiley and Sons
🌐
English
⚖ 535 KB
The effect of added nucleophiles (methanol and 1,4-butanediol) on the steady-state kinetics of a-chymotryptic hydrolysis of a series of -V-acetyl-L-amino acid methyl esters, R-CH(NHCOCH3)C(O)OCH3, has been studied. As a result, the rate and equilibrium consta.nts of the "elementary" steps of the enz