In vitro effects of tamoxifen on UM-SCC head and neck cancer cell lines: Correlation with the estrogen and progesterone receptor content
✍ Scribed by Thomas Carey; Reidar Grenman; Erkki Virolainen; Amnon Shapira
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- French
- Weight
- 612 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Eleven squamous-cell carcinoma (SCC) cell lines derived noma (SCC) cell lines derived from patients with head and from patients with head and neck cancer were tested together neck cancer (Virolainen et al., 1984). In that study, most of inhibition by tamoxifen. MCF-7 is known t o contain cytosolic expressed both ER and p g ~, whereas scc lines from other (AR) and glucocorticoid. We have previously reported the ER, PgR and AR contents of these I I head-and-neck SCC cancer PgR. Subsequently we examined the effects of tamoxifen on lines. Starting from day 3 or 4 after passage, cultures were the growth Of ERC laryngeal lines and On the MCF-7 fed daily with medium containing 5% dextran-charcoal-treated breast Cancer line (Shapira et al., 1986). While growth inhibifetal bovine serum (DS) and 0, I , 2.5,s. 7.5 or 10 PM tamoxifen. tion was observed with tamoxifen in both cell types, two Eight of the I I SCC lines and MCF-7 showed more than 60% patterns of recovery were observed. Breast cancer cells regrowth inhibition when fed with 5 P M tamoxifen. Of these 8 covered rapidly when fed with estradiol (E2) but more slowly SCC cell lines, 3 contained both ER and PgR, 4 contained Only when fed with D5 medium only, while UM-SCC-5 recovered PgR, and one contained neither receptor. The 3 cell lines that rapidly when fed with either ~~-~~~ medium or ~5 were not inhibited by tamoxifen failed to express either ER medium. This observation suggests that there may be two