In vitro cytotoxicity of CD8+ T cells in multi-drug-resistant tuberculosis. A preliminary report
✍ Scribed by Isabel SADA-OVALLE; Luis TORRE-BOUSCOULET; Rafael VALDEZ-VÁZQUEZ; Ricardo LASCURAIN
- Book ID
- 108965009
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 128 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1323-7799
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## Abstract The requirements for the conversion of CD8^+^ memory T cells into effector class I major histocompatibility complex (MHC) K^d^−restricted cytotoxic T (T~c~) cells __in vitro__ have been studied. Purified CD8^+^splenocytes from influenza A/WSN‐primed BALB/c (H‐2^d^) mice stimulated with
A novel fluorometric microculture cytotoxicity assay (FMCA), based on measurements of fluorescein diacetate (FDA) hydrolysis and DNA staining by Hoechst 33342, was used for drug sensitivity testing and detection of resistance reversal in acute lymphoblastic leukemia (ALL) cell lines. The 72-hr assay