In vitro culture with prednisolone increases BCL-2 protein expression in adult acute lymphoblastic leukemia cells
✍ Scribed by Tosi, Patrizia; Visani, Giuseppe; Ottaviani, Emanuela; Manfroi, Silvia; Tura, Sante
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 328 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0361-8609
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✦ Synopsis
The presence of BCL-2gene rearrangement has been detected also in cellular populations lacking the t(14;18) chromosomal translocation, such as B-lineage acute lymphobiastic leukemia (ALL) cells. It has been reported that overexpression of BCL-2 is related to resistance to glucocorticoid-induced apoptosis.
In this study, we aimed to evaluate whether in vitro culture with prednisolone (PDN) could modify the expression of BCL-2 protein. ALL cells from 21 patients were incubated for 72 hr with or without a minimally lethal (IC12) dose of PDN. In vitro culture with PDN did not affect the percentage of positive cells, even though the mean fluorescence index was signiflcantly increased (P = O.OOOl ), thus indlcating a higher level of protein production. These data could suggest a possible mechanism of drug resistance after treatment with PDN.