In vitro culture of sugarcane infected with maize streak virus (MSV)

✍ Scribed by J. P. Peros; E. Bonnel; B. Reynaud

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 919 KB
• Volume: 23
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00034424

✦ Synopsis

Young buds and leaf tissues of sugarcane cv. R 574 (a Saccharum hybrid), healthy or infected with Maize Streak Virus (MSV) were cultured in vitro. The success rate of the cultures from infected material is lower than that from healthy material. The symptoms of the disease were displayed on most of the plantlets produced from buds but only on 6% of the plantlets regenerated from calli. The indirect double-antibody sandwich ELISA technique using a monoclonal antibody makes it possible to identify virus-infected plantlets. In vitro, the infection results in the reduced growth of shoots.