✦ LIBER ✦
In vitro culture methods for transition stages of filarial parasites: Evidence that host factors are important for parasite development
✍ Scribed by Mary S. Cupp; Tovi Lehmann; Eddie W. Cupp
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 827 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1573-0603
No coin nor oath required. For personal study only.
✦ Synopsis
Simulium vittatum is a black fly that can the site where continued differentiation occurs. serve as a surrogate intermediate host for the filarial Medium conditioned by a vertebrate cell line parasite, Onchocerca lienalis. Use of media condi-(LLCMK,) supported vigorous culture of third-stage tioned by excised thoraces of S. vittatum led to the (L3) and fourth-stage larvae (L4), and provided an identification of a soluble chemical cue(s) used by environment for uncomplicated screening of therathe microfilariae to migrate to fly thoracic muscles, peutic drugs such as ivermectin.