In vitro contractile responses to acetylcholine in jejunal and colonic tissue from stressed rats

✍ Scribed by Ruben Stam; Alfons B.A. Kroese; Louis M.A. Akkermans; Victor M. Wiegant

• Book ID: 101302571
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 342 KB
• Volume: 19
• Category: Article
• ISSN: 0893-6609
• DOI: 10.1002/(sici)1520-6769(199611)19:3<161::aid-nrc176>3.0.co;2-5

✦ Synopsis

In order to investigate the mechanism for stress-induced sensitization of colonic motility responses in vivo, rats were exposed to a 15-minute session of repeated foot shocks, and contractile responses of proximal jejunal and colonic strips to non-cumulative doses of ' acetylcholine measured in vitro 14 days or 28 days later. No differences in dose-response relationships were found between intestinal strips from foot shocked rats and controls. Responses to the highest dose (lo4 M) of acetylcholine in the absence and presence of tetrodotoxin did not differ for any of the three experimental groups. Changes in cholinergic sensitivity in the gut are unlikely to underlie the increased colonic motility response to stress previously reported after a single foot shock experience.