The lysA gene of Escherichia coli has been cloned from a lambda transducing phage on various plasmids, present in different copy numbers in bacterial cells. Synthesis of the product of this gene, diaminopimelate (DAP)-decarboxylase, and its regulation have been studied. Expression does not follow a
In vitro construction of plasmids which result in overproduction of the protein product of the araC gene of Escherichia coli
β Scribed by Steffen, David ;Schleif, Robert
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 320 KB
- Volume
- 157
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Derivatives of the Escherichia coli drug resistance plasmid pMB-9 were constructed which contain the promoter from the lactose operon of E. coli fused to the araC gene of E. coli. E. coli possessing these plasmids contain about 50 times as much of the araC gene product as do cells with a wild-type araC gene and promotor.
π SIMILAR VOLUMES
Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins
From the specialized transducing bacteriophage lambdacysB, recombinant phages lambdacysB242 and lambdacysB257 have been obtained, each of which carries an amber mutation in the cysB cistron. A comparison of polyacrylamide gel electrophoretic profiles of labelled extracts from uv-irradiated bacteria
The gene secY (or prlA) is essential for protein export across the cytoplasmic membrane of Escherichia coli. The protein product of secY has been identified using the gene cloned under the control of the pL promoter of phage lambda in combination with the maxicell system. The protein was found to ha
The copy numbers of the expression plasmids (pRTF309+ and pMTC48), the repression plasmids (pc1857 and pRK248cI) and the protection plasmid (pEcoR4) in recombinant E. coli JM 103 were investigated. In the absence of the protection plasmid, the copy number of the expression plasmid dropped; in its pr