✦ LIBER ✦
In vitro cloning of a rat ascites hepatoma cell line, AH66, with special reference to alpha-fetoprotein synthesis
✍ Scribed by Yutaka Tsukada; Masahiko Mikuni; Hidematsu Hirai
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- French
- Weight
- 369 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Cell clones producing low amounts of alpha‐fetoprotein were isolated from AH66 cell line from an ascites hepatoma producing high amounts of AFP in vitro. The growth rate and the chromosome constitution were compared between clones producing high and low amounts of AFP. No significant correlation was found between the rate of AFP production and the chromosome number. A slight increase in host survival time was observed when the rats were transplanted with low‐AFP‐producing clones compared with high‐AFP‐producing clones.