In vitro cellular responses to bioerodible particles loaded with recombinant human bone morphogenetic protein-2
✍ Scribed by Puleo, D. A. ;Huh, W. W. ;Duggirala, S. S. ;DeLuca, P. P.
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 229 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Porous 50:50 poly(d,l lactide-co-glycolide) microspheres containing varying amounts of ''free'' recombinant human bone morphogenetic protein-2 (rhBMP-2) were evaluated for their ability to induce/enhance expression of osteoblastic characteristics by pluripotent mesenchymal cells in vitro. ''Free'' protein (F p ) is defined as protein present on the surface and within the porous matrix of the microspheres. Four preparations of bioerodible particles (BEP) were used: blank-without rhBMP-2; low F p -24 g of free rhBMP-2 per g of particles; medium F p -403 g/g; and high F p -884 g/g. C3H10T1/2 cells (C3H) and bone marrow stromal cells (BMC) were cultured with 1 mg of BEP for up to 4 weeks, and cell growth and expression of osteogenic responses were determined weekly. For both cell types, control cultures (neither BEP nor rhBMP-2) and cultures with blank BEP exhibited no or minimal osteoblastic characteris-tics. Compared to control and blank BEP cultures, C3H cells responded to particles having medium and high amounts of free rhBMP-2 with increased cell growth and alkaline phosphatase activity, but osteocalcin secretion and mineralization were not markedly influenced. Low F p BEP enhanced only the alkaline phosphatase activity of C3H cells. In contrast, although growth was not affected, rhBMP-2-loaded BEP increased alkaline phosphatase activity, osteocalcin secretion, and mineralization in BMC cultures in a dosedependent manner (i.e., blank < low < medium < high F p ).