In vitro cardiomyogenic differentiation of adipose-derived stromal cells using transforming growth factor-β1

✍ Scribed by So-Jung Gwak; Suk H. Bhang; Hee S. Yang; Sang-Soo Kim; Dae-Hee Lee; Soo-Hong Lee; Byung-Soo Kim

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 295 KB
• Volume: 27
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/cbf.1547

✦ Synopsis

Abstract

Transplanting stem cells differentiated towards a cardiac lineage can regenerate cardiac muscle tissues to treat myocardial infarction. In this study, we tested the hypothesis that transforming growth factor‐__β__1 (TGF‐__β__1) induces cardiomyogenic differentiation of adipose‐ derived stromal cells (ADSCs) in vitro. Rat ADSCs were cultured with TGF‐__β__1 (10 ng ml^−1^) for 2 weeks in vitro. ADSCs cultured without TGF‐__β__1 served as a control. The mRNA expression of cardiac‐specific gene was induced by TGF‐__β__1, while the control culture did not show cardiac‐specific gene expression. Immunocytochemical analyses showed that a small fraction of ADSCs cultured with TGF‐__β__1 for 2 weeks stained positively for cardiac myosin heavy chain (MHC) and α‐sarcomeric actin. Flow cytometric analyses showed that the proportion of cells expressing cardiac MHC increased with TGF‐__β__1. However, no mesenchymal differentiation (e.g., osteogenic and adipogenic differentiation) was detected other than cardiomyogenic differentiation. These results showed that TGF‐__β__1 induce ADSC cardiomyogenic differentiation in vitro, which could be useful for myocardial infarction stem cell therapy. Copyright © 2009 John Wiley & Sons, Ltd.