In vitro biological response to a self-adhesive resin cement under different curing strategies
✍ Scribed by Gabriela Q. M. Monteiro; Fábio B. Souza; Ricardo F. Pedrosa; Germana C. F. Sales; Célia Maria M. B. Castro; Simone N. Fraga; Bruno H. A. Galvão; Rodivan Braz
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 116 KB
- Volume
- 9999B
- Category
- Article
- ISSN
- 1552-4973
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Self‐adhesive resin cements represents a new approach in indirect restorative dentistry, although little is known about its biological properties. The influence of the curing strategy on the biological response of a self‐adhesive resin cement (Rely‐X Unicem/3M ESPE) was evaluated through methyltetrazolium (MTT) and nitric oxide (NOx) assays. Cylindrical specimens (n = 48) were prepared and cured according to the following strategies: photoactivation with halogen light‐curing unit (LCU) Demetron/Kerr, LED LCU Radii/SDI, and chemical activation. A control group with culture medium and a group with lipopolysaccharide, employed for cells stimulation, were used for comparisons (n = 8). After 24 h of direct contact between specimens and rat alveolar macrophages, the biological response was evaluated. Statistical evaluation of the data was performed with Kruskal‐Wallis and Dunn's test (p = 0.05). The MTT test showed that the specimens cured by halogen light and chemical activation provided higher alterations on cell metabolism. For LED‐cured specimens, cell viability was compatible to the results observed in the control group. For the NOx assay, all curing methods were cytotoxic to rat macrophages. Rely‐X Unicem demonstrated different cytotoxic effects according to the curing strategy employed. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010